MBᐩ + AA = LMB (leucomethylene blue, colorless) + DHA (dehydroascorbic acid 🛈)
About Methylene Blue (MBᐩ)
Methylene blue (methylthioninium chloride, Urolene Blue) was first identified in 1876 by Heinrich Caro, a German chemist. It has since been used in many applications:
- Methemoglobinemia, a blood disorder in which too little oxygen is delivered to your cells. MB increases the oxygen-carrying capacity of hemoglobin, the main component of red blood cells. It also acts as an electron shuttle, carrying electrons between proteins involved in the electron transport chain. By doing so, it helps increase the activity of the enzymes in the electron transport chain, allowing them to produce more ATP from the oxygen that is being taken up. This increased ATP production results in more oxygen delivery to tissues, thereby improving oxygen delivery to tissues.
- increases oxygen consumption of tissues using aerobic glycolysis {ref}
- malaria
- as an antidepressant
- antidote for cyanide poisoning
- treatment for carbon monoxide poisoning
- urinary tract infections
- vasoplegia
- septic shock
- analgesic
- in Alzheimer’s disease
- a titration indicator in a laboratory
- Nitric Oxide and estrogen antagonist
- can help reduce lactic acid
- Covid: 0.1mg as nebulization primary treatment, oral supportive. IV for severe cases. {ref} The reduced form of methylene blue (Leucomethylene) should be used for (oral) treatment.{ref}
- Methylene blue photodynamic therapy induces selective and massive cell death in human breast cancer cells {study}
🔶 Potential side effects include headache, vomiting, blue-tinted urine or stools, shortness of breath, and high blood pressure. Contraindicated for people with glucose-6-phosphate dehydrogenase (G6PD) deficiency.
MB is a potent monoamine oxidase inhibitor (MAOI). MB should NOT be combined with serotonin reuptake inhibitors {ref}
Impairment of histamine metabolism by monoamine oxidase inhibitors may allow the accumulation of histamine in the tissues from endogenous sources or from the intake in the diet.{ref}
Methylene blue at a dose of 15 mg/day for 3 weeks proved to be a strong antidepressant {ref}.
For regular or intermittent use, 100mcg to 1-2 milligrams of LeucoMB seems to be more than enough. LeucoMB is quite potent even at very low doses.
Avoid taking it with coffee or other beverages with plenty of caffeine. And as already mentioned above, don't take MB if you're on SSRI antidepressants.
Preparation of LMB
Approx 5 milligrams of MB (10 drops of a 1% MB solution) with approx 700 milligrams of AA.
 |
| 200ml of water with 10 drops MB 1% |
 |
| 10 minutes after I added 500 mg AA |
 |
| @ 20 minutes, looks like blue Gatorade |
 |
| added more AA (200 mg) |
 |
| 10 minutes later. Almost clear |
In a similar way, one could prepare a 15mg MB solution in approx 450ml of water, add approx 2000mg (2 grams) of AA and wait about 40 minutes for the water to turn clear.
Methylene blue at a dose of 15 mg/day has been found to be a potent antidepressant:
Glucose reduces methylene blue to leucomethylene blue (LMB) and is thereby oxidized to gluconic acid. Leucomethylene blue is oxidized again to methylene blue by oxygen.
MBᐩ + AA = LMB (leucomethylene blue, colorless) + DHA (dehydroascorbic acid)
Skip to Minute 01:00
 |
| 50 drops of 1% solution (approx 25 milligrams of MB) with 3,5 grams (3500 milligrams AA) |
in 500ML of water (0,5 Liters)
 |
| After 30 Minutes. |
Preparation 1 drop (0,5mg or 500mcg) of 1% Methylene Blue solution in a 30ml dropper bottle, mixed with Ascorbic Acid.
 |
| Measure cup with 30ml water |
 |
| Add to glass |
 |
| Add a drop of 1% MB |
 |
| Dissolve about 100mg of ascorbic acid to make the solution colorless |
 |
| Add to dropper bottle |
Similarly, 16 1% MB drops in 30ml of water dissolving approx 1 gram of ascorbic acid will make the solution turn almost colorless. That's 8mg MB in a 30ml bottle, and each full dropper approx 0,5mg (500mcg) Methylene Blue, or about 12,5mcg per drop, or 100mcg every 8 drops.
Personal experience taking MB + AA:
Note, I drink about 6-7 cups of coffee a day so this can be interfering with how I react to (leuco)MB (blood pressure).
Day 1: 1mg. felt a little agitated, like with too much caffeine. Clear urine the next day.
Day 2: 5mg. Drank the 5mg LMB/DHA solution around lunchtime but not all at once, same feeling as the day before, with a tingling feeling also. Greenish urine next morning and afternoon.
Day 3: 1mg late at night, tingling feeling followed by somnolence. Woke up with a bit of a hangover feeling. Slept about an hour longer than usual.
Day 4: 100 mcg (0,1 mg). Surprised to see greenish urine the next morning. I've read MB half-life is anywhere between 6 to 30 hours.
Day 5: 50mcg before bed. No greenish urine. Seems to reduce the onset of sleep for me at this low mcg dose. More REM sleep possibly.
Day 6: OFF. A big pimple appeared on my chin, never had this before, with water-like liquid coming out.
Day 7: OFF
Day 8: 50mcg before bed
Day 9: 50mcg before bed. Light greenish urine, even at this small dose.
REFERENCES
https://aacrjournals.org/amjcancer/article/24/1/80/679057/The-Effect-of-Methylene-Blue-on-the-Oxygen
https://pubmed.ncbi.nlm.nih.gov/28495375/ "MB efficiently stimulated SLP and restored the membrane potential in mitochondria also with the combined inhibition of Complex I and ATP synthase. ATP formed by SLP alleviated the energetic insufficiency generated by the lack of oxidative phosphorylation."
STIMULATION OF THE GLUCOSE OXIDATIVE PATHWAY IN HUMAN ERYTHROCYTES BY METHYLENE BLUE {ref}
.jpeg)
No comments:
Post a Comment